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Journal of Environmental Accounting and Management
António Mendes Lopes (editor), Jiazhong Zhang(editor)
António Mendes Lopes (editor)

University of Porto, Portugal

Email: aml@fe.up.pt

Jiazhong Zhang (editor)

School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China

Fax: +86 29 82668723 Email: jzzhang@mail.xjtu.edu.cn


Optimization of the Gram Staining Method Based on Superparamagnetic Magnetic Nanobeads

Journal of Environmental Accounting and Management 6(3) (2018) 263--271 | DOI:10.5890/JEAM.2018.09.007

Yuren Feng; Zihe Ren; Fuhua Wu; Annika Reichert; Yun Lu

School of Environment, Tsinghua University, Beijing, 100084, China

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Abstract

Gram staining is a widely used method for bacterial identification, where an alcohol burner is used as the fixed step, which requires a high level of proficiency, while the open flame is a potential fire hazard. A rapid, convenient and alternative method is also vailable that does not require a microscope or alcohol burner. This method utilizes the adsorption capacity of magnetic nanobeads. Ferriferous oxide beads are made by means of the co-precipitation method and adsorb bacteria in suspension liquid after being coated on the surface. The staining process omits the “heat” step and the need for a microscope, and distinguishes bacteria through the observation of the color of the liquid. According to our results, the behavior of this magnetic material performs well with various types of microbes. In particular, the color is clear and is easily distinguishable.

Acknowledgments

This work was supported by the National Key R&D Program of China (2016YFC0401405) and the National Key R&D Program of China for International Science & Innovation Cooperation Major Project between Governments (2016YFE0118800).

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