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Journal of Environmental Accounting and Management
António Mendes Lopes (editor), Jiazhong Zhang(editor)
António Mendes Lopes (editor)

University of Porto, Portugal


Jiazhong Zhang (editor)

School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China

Fax: +86 29 82668723 Email:

Removal of Dibutyl Phthalate and Its Effects on Bacterial Communities in Lab-scale Constructed Wetlands

Journal of Environmental Accounting and Management 7(1) (2019) 1--10 | DOI:10.5890/JEAM.2019.03.001

Xiaohui Wang$^{1}$, Jingdan Yang$^{1}$, Shuai Du$^{1}$, Yuan Yuan$^{2}$, Minghan Zhu$^{1}$, Yuan Li$^{1}$, Xiaobiao Zhu$^{1}$

$^{1}$ Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China

$^{2}$ College of Forestry, Henan Agriculture University, Zhengzhou, 450002, China

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The environmental release of phthalate acid esters (PAEs), and their potential health and environmental risks have attracted increasing concerns. The removal efficiencies of the most frequently identified PAE, dibutyl phthalate (DBP), and their effects on bacterial community structures were examined in two vertical flow constructed wetlands (VFCWs). In this study, the influent DBP concentration was about 600 μg/L and the removal rates reached above 61.1% during the study period. Catalase activity analysis showed that both treatment and control systems had significantly higher enzyme activities in the surface soil than subsurface substrate, and DBP significantly deteriorated the catalase activities (p < 0.05). Miseq sequencing data showed that DBP significantly increased the diversity of bacterial community (p < 0.05) and altered the overall bacterial community structure in wetland systems as revealed by Principal component analysis (PCA). The relative abundance of five genera, including Azohydromonas, Bdellovibrio, Desulfobulus, Roseomonas and Thiobacillus significantly decreased in the wetland at the present of DBP. However, four genera Propioniciclava, Geobacter, Paludibacter and Bacillus significantly increased under DBP. This study provides insights into our understanding the treatment performance of DBP in wetland systems, and their effects on the diversity and structure of bacterial communities under DBP in wetland systems.


This study was supported by the National Key Research and Development Program of China (2016YFC0401105), Beijing Municipal Science and Technology Project (Z171100002217029), and the Fundamental Research Funds for the Central Universities (JD1704).


  1. [1]  Ahuactzin-Pérez, M., Tlecuitl-Beristain, S., García-Dávila, J., Santacruz-Juárez, E., González-Pérez, M., Gutiérrez-Ruíz, M.C., and Sánchez, C. (2018), A novel biodegradation pathway of the endocrine-disruptor di(2-ethyl hexyl) phthalate by Pleurotus ostreatus based on quantum chemical investigation, Ecotoxicology and Environmental Safety, 147, 494-499.
  2. [2]  Bouali, M., Zrafi, I., Bakhrouf, A., Chaussonnerie, S., and Sghir, A. (2014), Bacterial structure and spatiotemporal distribution in a horizontal subsurface flow constructed wetland, AppliedMicrobiology and Biotechnology, 98(7), 3191-3203.
  3. [3]  Chang, J.J., Wu, S.Q., Liang, K., Wu, Z., and Liang, W. (2015), Comparative study of microbial community structure in integrated vertical-flow constructed wetlands for treatment of domestic and nitrified wastewaters, Environmental Science and Pollution Research, 22(5), 3518-3527.
  4. [4]  Chen, T.Y., Kao, C.M., Yeh, T.Y., Chien, H.Y., and Chao, A.C. (2006), Application of a constructed wetland for industrial wastewater treatment: a pilot-scale study, Chemosphere, 64(3), 497-502.
  5. [5]  Deng, Y., Zhang, P., Qin, Y., Tu, Q., Yang, Y., He, Z., Schadt, C.W., and Zhou, J. (2016), Network succession reveals the importance of competition in response to emulsified vegetable oil amendment for uranium bioremediation, Environmental Microbiology, 18(1), 205-218.
  6. [6]  Gledhill, W.E., Kaley, R.G., Adams, W.J., Hicks, O., Michael, P.R., Saeger, V.W., and Leblanc, G.A. (1980), An environmental safety assessment of butyl benzyl phthalate Environmental Science & Technology, 14(3), 301.
  7. [7]  Iasur-Kruh L, Hadar Y,Milstein D, Gasith A. andMinz D. (2010),Microbial population and activity in wetlandmicrocosms constructed for improving treated municipal wastewater Microbial Ecology 59(4), 700-709.
  8. [8]  Liang, Y.,Meggo, R., Hu, D., Schnoor, J.L., andMattes, T.E. (2015),Microbial community analysis of switchgrass planted and un-planted soilmicrocosms displaying PCB dechlorination, AppliedMicrobiology and Biotechnology, 99(15), 6515- 6526.
  9. [9]  Lin, Y.F., Jing, S.R., Lee, D.Y., Chang, Y.F., Chen, Y.M. and Shih, K.C. (2005), Performance of a constructed wetland treating intensive shrimp aquaculture wastewater under high hydraulic loading rate, Environmental Pollution, 134(3), 411-421.
  10. [10]  Lv, T., Carvalho, P.N., Zhang, L., Zhang, Y., Button, M., Arias, C.A., Weber, K.P., amd Brix, H. (2017), Functionality of microbial communities in constructed wetlands used for pesticide remediation: Influence of system design and sampling strategy, Water Research, 110, 241-251.
  11. [11]  Mackintosh, C.E., Maldonado, J.A., Ikonomou,M.G., and Gobas, F.A.P.C. (2006), Sorption of phthalate esters and PCBs in a marine ecosystem, Environmental Science & Technology, 40(11), 3481-3488.
  12. [12]  Majeed, K.A., Rehman, H.U., Yousaf, M.S., Zaneb, H., Rabbani, I., Tahir, S.K., and Rashid, M.A. (2017), Sub-chronic exposure to low concentration of dibutyl phthalate affects anthropometric parameters and markers of obesity in rats, Environmental Science and Pollution Research, 24(32), 25462-25467.
  13. [13]  Matamoros, V., García, J., and Bayona, J.M. (2005), Behavior of Selected Pharmaceuticals in Subsurface Flow Constructed Wetlands: A Pilot-Scale Study, Environmental Science & Technology, 39(14), 5449-5454.
  14. [14]  Nedashkovskaya,O.I., Balabanova, L.A., Zhukova,N.V., Kim, S.J., Bakunina, I.Y., and Rhee, S.K. (2014), Flavobacterium ahnfeltiae sp. nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga, Archives of Microbiology, 196(10), 745.
  15. [15]  Planelló, R., Herrero, O., Martinez-Guitarte, J.L., and Morcillo, G. (2011), Comparative effects of butyl benzyl phthalate (BBP) and di(2-ethylhexyl) phthalate (DEHP) on the aquatic larvae of Chironomus riparius based on gene expression assays related to the endocrine system, the stress response and ribosomes, Aquatic Toxicology, 105(1-2), 62-70.
  16. [16]  Soler-Llavina, S.M. and Ortiz-Zayas, J.R. (2017), Emergent contaminants in the wastewater effluents of two highly populated tropical cities, Journal of Water and Health, 15(6), 873-884.
  17. [17]  Shutes, R.B. (2001), Artificial wetlands and water quality improvement, Environment International, 26(56), 441-447.
  18. [18]  Sugawara, Y., Ueki, A., Abe, K. (2010), Propioniciclava tarda gen. nov., sp. nov., isolated from a methanogenic reactor treating waste from cattle farms, International Journal of Systematic and Evolutionary Microbiology, 61(Pt 9), 2298- 2303.
  19. [19]  Sun, B., Ko, K., Ramsay, J.A. (2011), Biodegradation of 1,4-dioxane by a Flavobacterium, Biodegradation, 22(3), 651-659.
  20. [20]  Xiao, Y., Chen, S.H., Gao, Y.Q., Hu, W., Hu, M., and Zhong, G. (2015), Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway, Applied Microbiology and Biotechnology, 99(6), 2849- 2859.
  21. [21]  Xu, D., Gu, J., Li, Y., Zhang, Y., Howard, A., Guan, Y., Li, J., and Xu, H. (2016), Purifying capability, enzyme activity, and nitrification potentials in December in integrated vertical flow constructed wetland with earthworms and different substrates, Environmental Science and Pollution Research, 23(1), 273-281.
  22. [22]  Wang, X., Zheng, Q., Yuan, Y., Hai, R. and Zou, D. (2017), Bacterial community and molecular ecological network in response to Cr 2 O 3 nanoparticles in activated sludge system, Chemosphere, 188, 10-17.
  23. [23]  Wang, X., Li, J., Liu, R., Hai, R.T., Zou, D.X., Zhu, X.B., and Luo, N. (2017), Responses of Bacterial Communities to CuO Nanoparticles in Activated Sludge System, Environmental Science & Technology, 51(10), 5368-5376.
  24. [24]  Wu, Y., Li, J., Yan, B., Zhu, Y., Liu, X., Chen, M., Li, D., Lee, C.C., Yang, X., and Ma, P. (2017), Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats Scientific Report, 13, 7.
  25. [25]  Yuan, S.Y., Huang, I.C. and Chang, B.V. (2010), Biodegradation of dibutyl phthalate and di-(2-ethylhexyl) phthalate and microbial community changes in mangrove sediment, Journal of Hazardous Materials, 184(13), 826-831.
  26. [26]  Zhou, Q.H.,Wu, Z.B., Cheng, S.P., He, F., and Fu, G.P. (2005), Enzymatic activities in constructed wetlands and di-n-butyl phthalate (DBP) biodegradation Soil Biology and Biochemistry, 37(8), 1454-1459.